Molecular Biologist and lab manager
I am in charge of the molecular biology section of the laboratory. I engineer most of the polyproteins that we use. We have a well established protocol for constructing polyproteins at the DNA level and then expressing them in bacteria. I use Talon columns and FPLC for protein purification. We struggle a bit with engineering AFM ready polyproteins. Not all of them give nice pulls. If so, I am the one that usually gets to re-engineer and/or re-express the proteins. I teach all the lab members how to do it and also I am happy to help other investigators by phone and email. I welcome inquiries.